Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2015, Volume 18, Issue 2

Identification of Yeasts Tested for Fermentation on Different Carbon Sources

Iuliana Diana Bărbulescu 1 , Mihaela Begea 2 , Mihai Frincu 3 , Radu Tamaian 4,5,6

1 Pharmacorp Innovation SRL, Unirii Avenue, 030138, Bucharest, Romania
2 University Politehnica of Bucharest, Faculty of Biotechnical Systems Engineering, Bucharest, Romania
3 Pharmacorp Innovation SRL, Bucharest, Romania
4 National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, Uzinei Street no. 4, PO Box Râureni 7, 240050, Râmnicu Vâlcea, Romania
5 Biotech Corp SRL, Râmnicu Vâlcea, Romania
6 University of Bucharest, Faculty of Physics, 3Nano-SAE Research Centre, Bucharest-Măgurele, Romania

*Corresponding author: Diana Iuliana Barbulescu, and Radu Tamaian, E-mail:

Published: October 2015


Yeasts have a high impact on food and beverage production. These micro-organisms can be isolated from different sources (e.g. soil, leaf, residual biomass resulting from winemaking process) and can be identified by different methods. Our approach describes one method for isolation and one method for identification of yeasts from Buzău (Romania) wine area. After identification, using a shotgun proteomics approach (MALDI-TOF mass spectrometry), of a Saccharomyces cerevisiae (National Center for Biotechnology Information, Bethesda MD, USA – NCBI ID: 4932) strain with a high economical value, we focused on cultivating this particular yeast on two type of carbon sources in order to obtain yeast biomass at micropilot level.


yeast, MALDI-TOF mass spectrometry, wine

Tag search yeast MALDI-TOF mass spectrometry wine