Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2016, Volume 19, Issue 1

Identification of New Isolates (Micro-Organisms) from Vineyard Epiphytic Microflora Using Maldi-Tof Mass Spectrometry

Iuliana Diana Bărbulescu 1 , Constanţa Mihai 2 , Mihaela Begea 2 , Mihai Frincu 3 , Liviu Grigorica 4 , Gabriel Roceanu 5 , Simona Ioana Marinescu 3 , Razvan Ionut Teodorescu 2 , Radu Tamaian 6,7,8

1 Pharmacorp Innovation SRL, Unirii Avenue, 030138, Bucharest, Romania
2 University of Agronomic Sciences and Veterinary Medicine Bucharest, Romania
3 Pharmacorp Innovation SRL, Bucharest, Romania
4 Bevitech SRL, Bucharest, Romania
5 Carl Reh Winery SRL, Bucharest
6 National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, Uzinei Street no. 4, PO Box Râureni 7, 240050, Râmnicu Vâlcea, Romania
7 Biotech Corp SRL, Râmnicu Vâlcea, Romania
8 University of Bucharest, Faculty of Physics, 3Nano-SAE Research Centre, Bucharest-Măgurele, Romania

*Corresponding author: Diana Iuliana Barbulescu, and Radu Tamaian, E-mail:

Published: May 2016


This paper presents the identification of new isolates (micro-organisms) from mature grapes berries & leaves of specific grape varieties and soil from Oprișor area, using MALDI-TOF mass spectrometry. Red grape varieties taken in study were: Cabernet Sauvignon, two Merlot varieties and Shiraz. In the previous study we identified yeast by MALDI-TOF from this wine area. For each variety of red grapes, leaves and soil samples have been also studied. The main micro-organisms from soil were Bacillus sp. from Cabernet area. From Shiraz and Merlot grapes samples the MALDI-TOF score value was 1.26 respectively 1.552, indicating a not reliable identification using MALDI-TOF mass spectrometry. These samples have been also characterized from microscopic and macroscopic point of view. Part of the samples which did not give reliable results by MALDI-TOF mass spectrometry will be the object for the next studies for more precise identification using techniques of molecular taxonomy.


MALDI-TOF mass spectrometry, wines, yeast, bacteria

Tag search MALDI-TOF mass spectrometry wines yeast bacteria