Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2019, Volume 22, Issue 1
Pages 103-112

Identification and analysis of tanins in extracts from walnuts green fruits and leaves

Claudia Cobzaru , Alice-Nicoleta Serban , Corina Cernatescu *

“Gheorghe Asachi” Technical University of Iasi, Faculty of Chemical Engineering and Environmental Protection D. Mangeron, 71, 70050, Romania

*Corresponding authors: Corina Cernatescu, E-mail:

Published: 2019


This study aims to identify and analyze the tannins from walnut extract obtained by discontinuous methods (infusion and maceration) and continuous methods (Soxhlet extraction). Experimental data show that walnut extracts obtained by discontinuous processes have a higher yield and a much higher content of tannins than those obtained by continuous processes. The walnut samples were used in cosmetic formulation of a shampoo.


  • Capparucci C, Gironi F, Piemonte V.
    Tannins Extraction from Walnuts Residues
    Chem. Eng., 24, 2011, 469-474

  • Cernatescu C.,
    Cosmetic technology. Laboratory Guide
    Ed. PIM, 2016

  • Cheng R.,
    Extraction technology and dyeing properties of walnut green husk pigment
    WUST, 2017

  • Cobzaru C., 2014
    Natural Extracts: Particularities, Processes, Uses
    Ed. PIM, Iasi Cobzaru C., Horoba E., 2011, Natural Extracts. Laboratory Guide, Ed. PIM, Iasi

  • Cosmulescu S., Trandafir I., Achim Gh., Botu M., Baciu A., Gruia M.
    Phenolics of Green Husk in Mature Walnut Fruits
    Not. Bot. Hort. Agrobot. Cluj 38 (1), 2010, 53-56

  • Badi K. A., Khan A.S.
    Formulation, evaluation and comparison of the herbal shampoo with the commercial shampoos
    Beni-Suef University, J. Basic Appl. Sci., 3, 2014, 301-305

  • Baran R., Maibah HI.
    Cosmetic dermatology in children. Text book of cosmetic
    2 end, Ed. CRC Press, 1998, 507-508

  • Faker El-Din H.M., El-Messery T.M., Mehanna N.S., Ali A.A-E., Hassan Z.M.R., Amarowicz R.
    Interaction between some plants tannins and milk protein
    Intern. J. Food Nutrit. Sci., 4 (1), 2015, 16-20

  • Han M.M., Wang C.Y., Wang F., Pang M.X., Qu C., Qi J.H.
    Extraction and Dyeing Propertie of Tannin from Walnut Green Husk
    IOP Conf. Series: Earth and Environ. Sci., 1998, 2018

  • Jakopič J.R., Štampar F.
    Extraction of phenolic compounds from green walnut fruits in different solvents
    Acta Agricult. Slovenica, 93 (1), 2009, 11-15

  • Li H.Y., Han J.Q., Li Z.X.
    Extraction and separation of effective chemical constituents from walnut green husk
    Xiandai Horticult.15, 2012, 9-11

  • Ungureanu Ion C.
    Walnut culture (Juglans regia L.)
    Ed. Bren, Bucuresti, 2003

  • Soares S., Mateus N., de Freitas V.
    Interaction of different polyphenols with bovine serum albumin (BSA) and human salivary a-amylase (HSA) by fluorescence quenching
    J. Agric. Food Chem. 55, 2007, 6726–6735

  • Timko M.M.
    Walnut in Moldova
    Ed. Stat Moldova, Chisinau, 1965

  • Wang H., Li Y.X., Liu J.H.
    Ultrasonic assisted extraction of tannin in walnut green husk
    Mod. Agric. Sci. Technol., 8, 2011, 81-85

  • Keywords

    tannins, walnut extracts, tincture, infusion

    Tag search tannins walnut extracts tincture infusion