Smart Energy and Sustainable Environment , ISSN 2668-957X
2021, Volume 24, Issue 2
Pages 89-96


Carmen Varlam * , Diana Bogdan , Irina Vagner , Diana Costinel

National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, Uzinei Street no. 4, PO Box Râureni 7, 240050, Râmnicu Vâlcea, Romania

*Corresponding author: Carmen Varlam, E-mail:

Received 22 July 2021; Received in revised form 9 September 2021; Accepted 4 October 2021; Available online 9 November 2021


In order to validate the laboratory routine procedure of tritium activity concentration determination in tissue free water of environmental samples, parallel measurements were performed in water extracted from wild vegetation by two methods: azeotropic distillation and lyophilization (recommended by a French standard method). The sample humidity was established using the drying procedure to constant mass of the sample, and isotopic effect that may occur during lyophilization procedure was investigated by deuterium ratio determination in sample and extracted water. Traces of toluene in extracted water by azeotropic distillation made deuterium ration determination useless.


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Tritium, deuterium, azeotropic distillation, liophilization

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