Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2003, Volume 6, Issue 11+12

The Characterization of Regenerative Processes on Grapevine Culture Under DDW Influence

I. Stefanescu 1 , Al. Teodorescu 2 , Daniela Giosanu 3 , Emilia Visoiu 2 , Elena Buciumeanu 2 , Carmen Bejan 2 , Carmen Florentina Popescu 2

1 Institute of Cryogenics and Isotope Separation, Rm. Valcea
2 Research Station for Viticulture and Enology, Ştefăneşti - Argeş
3 University of Pitesti, Romania

*Corresponding author: Ioan Stefanescu, e-mail:

Published: October 2003


In order to study the influence of DDW on regenerative processes on grapevine, was made an explants culture, prelevated from plants belong to Feteasca alba and Feteasca Neagra sorts, which was growth on medium with DDW, comparative to usual medium. During three subculture (90-100 days of culture), the influence of DDW on the regenerative processes and the biochemical composition of biological material were observed. It was investigated the possibility of DDW use like sterile agent for explants (in initialization stage) and in treatment against mould (in acclimatization stage). Without exclude the endogen factor, our observations on biological material come from in vitro, demonstrated a positive influence of DDW, by the crease of multiplication and acclimatization rate.


Grapevine, in vitro culture, deuterium depleted water (DDW).

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