Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2014, Volume 17, Issue 2

HPLC Determination of Oleanolic and Ursolic Acid in Apples and Apple Pomace

Elisabeta-Irina Geana 1 , Roxana Elena Ionete 1 , Alexandru Ciocarlan 2* , Aculina Aricu 2 , Ala Fulga 2 , Nicon Ungur 2 , Marina Podogova 3 , Diana Nikolaeva 3

1 National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, Uzinei Street no. 4, PO Box Râureni 7, 240050, Râmnicu Vâlcea, Romania
2 Institute of Chemistry, 3, Academiei str., Chisinau MD 2028, Republic of Moldova
3 Scientific&Practical Institute of Horticulture, Viticulture and Food Technology, Laboratory of Food Technology, Vieru str. 59, MD-2070, Chisinau, Moldova

*Corresponding author: Alexandru Ciocarlan, E-mail:

Published: October 2014


Four different apples cultivars (Semirenko, Golden, Idared and Vagner) and two samples of apple pomace resulted from industrial juice production were subjected to immersion, Soxhlet and supercritical extractions in order to perform full extraction of ursolic (UA) and oleanolic acids (OA) from the mentioned sources. A simple and accurate RP-HPLC method for the determination of UA and OA in extracts obtained by different extraction methods was developed. According to HPLC analyses cuticular extracts from apple peels showed high contents of UA (398.50-541.91 mg/g) and OA (145.72-186.71 mg/g) which are present in a mixture from 2.2:1 to 3.7:1 ratios. The extracts obtained from pomace by Soxhlet extraction are much richer in triterpenic acids with respect to those obtained with subcritical fluid extraction. Both extraction methods proved that apple pomace is a suitable source of bioactive compounds such as ursolic and oleanolic acids.


HPLC; oleanolic acid; ursolic acid, triterpenes, apples, pomace.

Tag search HPLC oleanolic acid ursolic acid triterpenes apples pomace