SYNTHESIS OF TRITIUM LABELLED THYMIDINE AND URIDINE
1 National Institute for Physics and Nuclear Engineering, Magurele, Romania
2 “Horia Hulubei” National Institute for Physics and Nuclear Engineering IFIN HH, Magurele, Ilfov, Romania
3 “Horia Hulubei” National Institute of Nuclear Physics and Engineering, Bucharest
*Corresponding author: Cristian Postolache, E-mail: cristip@nipne.ro
Received 21 December 2021 Received in revised form 14 March 2022 Accepted 23 March 2022 Available online 04 April 2022OBSERVATION: The article is presented to the 23rd International Conference “New Cryogenic and Isotope Technologies for Energy and Environment" EnergEn 2021, Baile Govora, Romania, October 26-29, 2021
Abstract
This study present tritium labeling methods for uridine and thymidine in the methyl position. The synthesis of tritiated thymidine has been performed using two different methods: catalytic hydrogenation of brominated derivative and respectively of hydroxymethyl deoxiuridine.
The tritiated uridine has been synthesized by catalytic hydrogenation of 5-I Uridine.
The labelled compounds were purified by Thin Layer Chromatography and characterized by determination of radioactive concentration, chemical concentration and radiochemical purities.
The obtained tritiated thymidine and uridine have been used for in vitro studies for evaluation of radiation effects in cell cultures.
References
Matei L., Postolache C., Bubueanu G., Podina C. (2008), Synthesis of Labeled Compounds using Recovered Tritium from Expired Beta Light Sources, Fusion Sci Technol, 54: 643, DOI: 10.13182/FST08-A1897
Matei L., Neagoe I, Postolache C, Tanase V, Bubueanu G, Manda G (2010), In vitro and in vivo radiometrical studies evaluation of new nucleotide anologue behavior, J Labelled Comp Radiopharm, 53: 294
Postolache C., Tanase C., Matei L., Serban V. (2007), Synthesis of [5-3H] Uracil Nucleoside Analogue, J Labelled Comp Radiopharm, 50: 609, DOI: https://doi.org/10.1002/jlcr.1305
Postolache C., Matei L., Tanase C., Bubueanu G (2010), Synthesis of E-275 Class Nucleoside Analogue by Isotope Exchange Technique, J Labelled Comp Radiopharm, 53: 461
Tuta C. S., Postolache C., Fugaru V., Bubueanu G., Manea S., Bercea S. (2015), Facility for Removal and Determination of Labile Tritium, Acta Phys Pol A, 127: 895-897, DOI: 10.12693/APhysPolA.127.895
Keywords
Tritium, labelled compounds, catalytic hydrogenation, thymidine, uridine.
Tag search Tritium labelled compounds catalytic hydrogenation thymidine uridine
ARCHIVE
ISSN 1582-2575
Show more...