Progress of Cryogenics and Isotopes Separation , ISSN: 1582-2575
2010, Volume 13, Issue 2

Using Cleland's Reagent for Assessing the Pro-Oxidant Activity Oof Particulate Matter PM10 and PM2.5

Monica Mateescu 1 , Liana Simona Sbirna 2 , Anisoara Preda 3 , Sebastian Sbirna 4

1 University of Craiova, Faculty of Chemistry, 107I Calea Bucuresti, Craiova, Romania
2 University of Craiova, Faculty of Chemistry, 1071 Calea Bucuresti, 2000478 Craiova, Romania
3 National Institute for Research and Development for Cryogenics and Isotopic Technologies - ICIT Rm. Valcea, Uzinei Street no. 4, P.O. Box Raureni 7, 240050, Ramnicu Valcea, Romania
4 Dolj Environment Protection Agency

*Corresponding author: Simona Sbirna, e-mail:

Published: October 2010


Exposure to ambient particulate matter (PM) has been associated with a number of adverse health effects. Studies have suggested that such adverse health effects may derive from oxidative stress, initiated by the formation of reactive oxygen species (ROS) within affected cells.

The present study aims to assess physical characteristics and chemical compositions of PM and to correlate the results to their redox activity. PM10 (mass aerodynamic diameter ≤10 μm) and PM2.5 (mass aerodynamic diameter ≤2.5 μm) were collected in an urban area (Craiova city). The concentrations of organic carbon (OC) and elemental carbon (EC) were measured.

The Cleland’s reagent was used to measure the redox activity of PM.

Results show that the concentration of OC is greater than the one of EC (the volatile organic compounds are responsible for this) and that PM2.5 have higher concentrations of OC and EC than PM10.


Air quality, particulate matter (PM10, PM2.5), Cleland's reagent (DTT), organic carbon, elemental carbon.

Tag search Air quality particulate matter PM10 PM25 Cleland reagent DTT organic carbon elemental carbon